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Site-directed mutagenesis is used to generate mutations that may produce a rationally designed protein that has improved or special properties (i.e.protein engineering). Investigative tools – specific mutations in DNA allow the function and properties of a DNA sequence or a protein to be investigated in a rational approach. Furthermore ...
Patients with low activity (10% prevalence) or especially absent activity (prevalence 0.3%) are at a heightened risk of drug-induced bone marrow toxicity due to accumulation of the unmetabolised drug. Reuther et al. found that about 5% of all thiopurine therapies will fail due to toxicity. This intolerant group could be anticipated by routine ...
The location of a transversion mutation on a gene coding for a protein correlates with the extent of the mutation. If the mutation occurs at a site that is not involved with the shape of a protein or the structure of an enzyme or its active site, the mutation will not have a significant effect on the cell or the enzymatic activity of its proteins.
A mutation may occur to replace a tyrosine (which needs to be phosphorylated in order to activate the protein) with an aspartic acid (which would not need to be phosphorylated). In a laboratory setting, the use of recombinant proteins to artificially introduce phosphomimetics is a common tool for studying phosphorylation and protein activation.
A popular idea, that has failed to gain significant experimental support, is the idea that mutation, as distinct from DNA damage, is the primary cause of aging. Mice defective in the mutL homolog Pms2 have about a 100-fold elevated mutation frequency in all tissues, but do not appear to age more rapidly. [38]
4437 17686 Ensembl ENSG00000113318 ENSMUSG00000014850 UniProt P20585 P13705 RefSeq (mRNA) NM_002439 NM_010829 NM_001311120 RefSeq (protein) NP_002430 NP_001298049 NP_034959 Location (UCSC) Chr 5: 80.65 – 80.88 Mb Chr 13: 92.35 – 92.49 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse DNA mismatch repair protein, MutS Homolog 3 (MSH3) is a human homologue of the bacterial mismatch ...
Bosses are posting ‘ghost jobs’ that don’t exist. Here are 3 ways to spot a listing that isn’t real
Transposon mutagenesis is much more effective than chemical mutagenesis, with a higher mutation frequency and a lower chance of killing the organism. Other advantages include being able to induce single hit mutations, being able to incorporate selectable markers in strain construction, and being able to recover genes after mutagenesis. [ 2 ]