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Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. [1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis , [ 2 ] Francisella and Nocardia spp .
Yeast extract agar is a growth medium containing yeast extract. It may refer to: The nonselective yeast extract agar of Windle Taylor; YM (selective medium), for yeasts and molds; Buffered charcoal yeast extract agar, for Legionella
The detection of Legionella typically requires growing them on buffered charcoal yeast extract agar. As Legionella growth requires cysteine and iron , it cannot grow on other common lab media. To detect Legionella in water, it is first concentrated, then inoculated into charcoal yeast extract agar containing selective agents that prevent the ...
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
The yeast/meat extracts and peptone provide sources of carbon, nitrogen, and vitamins for general bacterial growth. The yeast extract also contains vitamins and amino acids required by Lactobacilli. Polysorbate 80 is a surfactant which assists in nutrient uptake by Lactobacilli.
The bacterium was first discovered in 1982 by R. L. Tyndall and C. B. Duncan, who were a part of D.J. Brenner's team that discovered ten new species of Legionella. [1] The isolation process initiated after collecting water samples and transferring them into guinea pig tissues before plating them onto buffered charcoal yeast extract agar. [1]