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Enriched media contain the nutrients required to support the growth of a wide variety of organisms, including some of the more fastidious ones. They are commonly used to harvest as many different types of microbes as are present in the specimen. Blood agar is an enriched medium in which nutritionally rich whole blood supplements the basic ...
Enrichment culture is the use of certain growth media to favor the growth of a particular microorganism over others, enriching a sample for the microorganism of interest. . This is generally done by introducing nutrients or environmental conditions that only allow the growth of an organism of inte
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
Chocolate agar showing Francisella tularensis colonies Comparison of two culture media types used to grow Neisseria gonorrhoeae bacteria. Known as overgrowth, the nonselective chocolate agar medium on the left, due to its composition, allowed for the growth of organismal colonies other than those of N. gonorrhoeae, while the selective Thayer–Martin medium on the right, containing ...
This medium has a variety of uses in microbiological investigations. The current formulation incorporated these later modifications: The primary value of Loeffler medium is in the growth and morphological characterization of members of the genus Corynebacterium.
Streak plates of several bacterial species on nutrient agar plates. Nutrient agar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms.
The term 'defined media' can also be used to describe this type of media. Media formulations containing the media supplement B27 (supplied by Invitrogen ) are often erroneously referred to as chemically defined media (e.g. Yao et al., 2006) despite this product containing bovine serum albumin (Chen et al., 2008) using the above definitions this ...
Alternative culture media [ edit ] While LJ medium is the most popular means of culturing mycobacteria, as recommended by the International Union against Tuberculosis, several alternative media have been investigated.