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Starting in 1994, light sheet fluorescence microscopy was developed as orthogonal plane fluorescence optical sectioning microscopy or tomography (OPFOS) [4] mainly for large samples and later as the selective/single plane illumination microscopy (SPIM) also with sub-cellular resolution. [5] This introduced an illumination scheme into ...
Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome. Many different techniques for optical sectioning are used and several microscopy techniques are specifically ...
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy. Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.
Microscopy. Scanning electron microscope image of pollen (false colors) Microscopic examination in a biochemical laboratory. Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). [1]
Light sheet fluorescence microscopy or selective plane imaging microscopy (SPIM) uses illumination that is done perpendicularly to the direction of observation, by using a thin sheet of (laser) light. Under certain conditions, this illumination principle can be combined with fluorescence correlation spectroscopy, to allow spatially resolved ...
Structured illumination light sheet microscopy (SI-LSM) is an optical imaging technique used for achieving volumetric imaging with high temporal and spatial resolution in all three dimensions. It combines the ability of light sheet microscopy to maintain spatial resolution throughout relatively thick samples with the higher axial and spatial ...
Dispersion staining. The optical properties of all liquid and solid materials change as a function of the wavelength of light used to measure them. This change as a function of wavelength is called the dispersion of the optical properties. The graph created by plotting the optical property of interest by the wavelength at which it is measured ...
This was demonstrated, for imaging and microscopy applications, in 1993. [6] [10] Alternative descriptions of this type of extremely elongated illumination include the terms line illumination, linear illumination, thin light sheet illumination (in light sheet microscopy), and plane illumination (in selective plane illumination microscopy).