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UV-Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.
This is similar to the situation in a hydrogen flame. Arc lamps made with ordinary light-hydrogen provide a very similar UV spectrum to deuterium, and have been used in UV spectroscopes. However, lamps using deuterium have a longer life span and an emissivity (intensity) at the far end of their UV range which is three to five times that of an ...
The cuvette is filled with sample, light is passed through the sample and intensity readings are taken. The slope spectroscopy technique can be applied using the same methods as in absorption spectroscopy. With the advent of accurate linear stages, variable pathlength absorption spectroscopy is easily applied experimentally.
This method requires a spectrophotometer capable of measuring in the UV region with quartz cuvettes. [3]: 135 Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5]
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
With the aid of these rules the UV absorption maximum can be predicted, for example in these two compounds: [8] In the compound on the left, the base value is 214 nm (a heteroannular diene). This diene group has 4 alkyl substituents (labeled 1,2,3,4) and the double bond in one ring is exocyclic to the other (adding 5 nm for an exocyclic double ...
With this source, Turner's group obtained an energy resolution of 0.02 eV. Turner referred to the method as "molecular photoelectron spectroscopy", now usually "ultraviolet photoelectron spectroscopy" or UPS. As compared to XPS, UPS is limited to energy levels of valence electrons, but measures them more accurately.
Diffuse reflectance spectroscopy, or diffuse reflection spectroscopy, is a subset of absorption spectroscopy. It is sometimes called remission spectroscopy . Remission is the reflection or back-scattering of light by a material, while transmission is the passage of light through a material.