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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
In molecular biology, quantitation of nucleic acids is commonly performed to determine the average concentrations of DNA or RNA present in a mixture, as well as their purity. Reactions that use nucleic acids often require particular amounts and purity for optimum performance. To date, there are two main approaches used by scientists to ...
Photoexcitation is the first step in a photochemical process where the reactant is elevated to a state of higher energy, an excited state.The first law of photochemistry, known as the Grotthuss–Draper law (for chemists Theodor Grotthuss and John W. Draper), states that light must be absorbed by a chemical substance in order for a photochemical reaction to take place.
An example of spectroscopy: a prism analyses white light by dispersing it into its component colors. Spectroscopy is the field of study that measures and interprets electromagnetic spectra. [1] [2] In narrower contexts, spectroscopy is the precise study of color as generalized from visible light to all bands of the electromagnetic spectrum.
Ultraviolet spectroscopy has wide applicability in molecular biology, particularly the study of photosynthesis. [68] It has been used to study a wide variety of flowering plants and ferns [69] by researchers in departments of biology, plant physiology and agriculture science as well as molecular genetics. [70]
It is the link between the electrochemistry and the UV-Vis absorption spectroscopy. [3] Devices to conduct the radiation beam: lenses, mirrors and/or optical fibers. The last ones conduct electromagnetic radiation over great distances with hardly any losses.
In modern spectrographs in the UV, visible, and near-IR spectral ranges, the spectrum is generally given in the form of photon number per unit wavelength (nm or μm), wavenumber (μm −1, cm −1), frequency (THz), or energy (eV), with the units indicated by the abscissa.