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Refractive index and extinction coefficient of thin film materials. A. R. Forouhi and I. Bloomer deduced dispersion equations for the refractive index, n, and extinction coefficient, k, which were published in 1986 [1] and 1988. [2] The 1986 publication relates to amorphous materials, while the 1988 publication relates to crystalline.
The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. [1] It is a quick and accurate [2] spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins.
List of refractive indices. Refraction at interface. Many materials have a well-characterized refractive index, but these indices often depend strongly upon the frequency of light, causing optical dispersion. Standard refractive index measurements are taken at the "yellow doublet" sodium D line, with a wavelength (λ) of 589 nanometers.
Complementing the Laporte rule, is the spin-selection rule, which forbids transitions that involve changes in spin state. Violations of both the Laporte and spin-selection rules results in particularly low extinction coefficients. Illustrative of this combined effect is the faintness of even concentrated solutions of octahedral Mn(II) and Fe ...
Bovine serum albumin (BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard in lab experiments. The nickname "Fraction V" refers to albumin being the fifth fraction of the original Edwin Cohn purification methodology that made use of differential solubility characteristics of plasma proteins.
Extinction coefficient refers to several different measures of the absorption of light in a medium: Attenuation coefficient, sometimes called "extinction coefficient" in meteorology or climatology. Mass extinction coefficient, how strongly a substance absorbs light at a given wavelength, per mass density. Molar extinction coefficient, how ...
The suffix "R" in the name of Coomassie brilliant blue R-250 is an abbreviation for "red" as the blue colour of the dye has a slight reddish tint. For the "G" variant the blue colour has a more greenish tint. The "250" originally denoted the purity of the dye. The colour of the two dyes depends on the acidity of the solution.
Bis (trimethylsilyl)acetamide. Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Bis (trimethylsilyl)acetamide (BSA) is an organosilicon compound with the formula MeC (OSiMe3)NSiMe3 (Me = CH 3). It is a colorless liquid that is soluble in diverse organic solvents, but reacts ...