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Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology. The term culture can also refer to the microorganisms being grown. Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both.
Counterstain, which is usually positively charged safranin or basic fuchsine, is applied last to give decolorized gram-negative bacteria a pink or red color. [3] [20] Both gram-positive bacteria and gram-negative bacteria pick up the counterstain. The counterstain, however, is unseen on gram-positive bacteria because of the darker crystal ...
Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. It provides an in vitro model of the tissue in a well defined environment which can be easily manipulated and analysed. In animal tissue culture, cells may be grown as two-dimensional monolayers (conventional culture) or within fibrous ...
Biology portal; To get a detailed description of the various methods see protein methods and nucleic acid methods. Besides the aforementioned groups of techniques, biotechnology also includes plant tissue culture methods, animal cell culture methods, and microbial fermentation methods.
This characteristic, in combination with other techniques makes it a useful tool in clinical microbiology laboratories, where it can be important in early selection of appropriate antibiotics. [ 8 ] On most Gram-stained preparations, Gram-negative organisms appear red or pink due to their counterstain.
This page was last edited on 7 September 2021, at 10:53 (UTC).; Text is available under the Creative Commons Attribution-ShareAlike 4.0 License; additional terms may apply.
Instrument Uses Autoclave: used for sterilization of glass ware and media Auto-destruct syringes: specimen collection Bijou bottle: a cylindrical small glass bottle with a screw cap used as a culture medium holder
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
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