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Sirius Red F 3B (Direct Red 80) is an azo dye primarily used in staining methods for collagen and amyloid. [1] It has the molecular formula C 45 H 26 N 10 Na 6 O 21 S 6.. In histology, sirius red staining is used in various domains of diagnostic to observe fibrosis levels in a lot of cases of inflammation induced by cancer, vascular or metabolic pathologies.
The recipes emerged from Claude L. Pierre Masson's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells from surrounding connective tissue. Most recipes produce red keratin and muscle fibers, blue or green collagen and bone, light red or pink cytoplasm, and dark brown to black cell ...
The first staining protocol that was described as "trichrome" was Mallory's trichrome stain, which differentially stained erythrocytes to a red colour, muscle tissue to a red colour, and collagen to a blue colour. Some other trichrome staining protocols are the Masson's trichrome stain, Lillie's trichrome, and the Gömöri trichrome stain.
Spindle cell lipoma Angiosarcoma Dermatofibrosarcoma protuberans Atypical fibroxanthoma Pleomorphic fibroma Intravascular large B-cell lymphoma: CD38: Plasma cell: Multiple myeloma Plasmablastic lymphoma Pagetoid reticulosis: CD44: Merkel cell carcinoma: CD56: Extranodal natural killer cell lymphoma Merkel cell carcinoma: CD68: Benign cephalic ...
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
It is similar to Masson's trichrome stain, but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940. [ 1 ] It is applied by submerging the fixated sample into the following three solutions: [ 2 ] Weigert's iron hematoxylin working solution, Biebrich scarlet solution, and Fast Green FCF solution.
Main staining types when using hematoxylin and eosin (H&E). A Basophil granulocyte stains dark purple upon H&E staining. Basophilic is a technical term used by pathologists. It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye.
This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with Light Green SF yellowish or methyl blue. It can also be used to identify growing bacteria. [3]