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Fluorescence Loss in Photobleaching (FLIP) is a fluorescence microscopy technique used to examine movement of molecules inside cells and membranes. A cell membrane is typically labeled with a fluorescent dye to allow for observation.
A simplified Jablonski diagram illustrating the change of energy levels.. The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely ...
A biological network is a method of representing systems as complex sets of binary interactions or relations between various biological entities. [1] In general, networks or graphs are used to capture relationships between entities or objects. [1]
Biological network inference is the process of making inferences and predictions about biological networks. [1] By using these networks to analyze patterns in biological systems, such as food-webs, we can visualize the nature and strength of these interactions between species, DNA, proteins, and more.
GenBank (National Center for Biotechnology Information) DDBJ (Japan), GenBank (USA) and European Nucleotide Archive (Europe) are repositories for nucleotide sequence data from all organisms . All three accept nucleotide sequence submissions, and then exchange new and updated data on a daily basis to achieve optimal synchronisation between them.
To study structures within living cells, in combination with fluorescence, one can utilize recombinant proteins containing fluorescent protein domains, e.g., green fluorescent protein (GFP). The GFP-technique involves altering the genetic information of the cells.
The DNA double helix with a cytosine base flipped out 180° DNA base flipping, or nucleotide flipping, is a mechanism in which a single nucleotide base, or nucleobase, is rotated outside the nucleic acid double helix. [1]
Cover of the first edition. Biology Today is a college-level biology textbook that went through three editions in 1972, 1975, and 1980. The first edition, published by Communications Research Machines, Inc. (CRM) and written by a small editorial team and large set of prominent "contributing consultants", is notable for its lavish illustrations and its humanistic approach.