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Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [1][2][3] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the reversed ...
The mobile phase consists of the sample being separated/analyzed and the solvent that moves the sample through the column. In the case of HPLC the mobile phase consists of a non-polar solvent(s) such as hexane in normal phase or a polar solvent such as methanol in reverse phase chromatography and the sample being separated. The mobile phase ...
In reversed phase chromatography, the most polar compounds elute first with the more nonpolar compounds eluting later. The mobile phase is generally a mixture of water and miscible polarity-modifying organic solvent, such as methanol, acetonitrile or THF. Retention increases as the fraction of the polar solvent (water) in the mobile phase is ...
Partition chromatography uses a retained solvent, on the surface or within the grains or fibers of an "inert" solid supporting matrix as with paper chromatography; or takes advantage of some coulombic and/or hydrogen donor interaction with the stationary phase. Analyte molecules partition between a liquid stationary phase and the eluent.
Paper chromatography is an analytical method used to separate coloured chemicals or substances. [1] It is now primarily used as a teaching tool, having been replaced in the laboratory by other chromatography methods such as thin-layer chromatography (TLC). The setup has three components. The mobile phase is a solution that travels up the ...
Thin-layer chromatography. Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. [1] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [2] This is called the stationary phase. [2] The sample is deposited on the plate, which ...
Any polar chromatographic surface can be used for HILIC separations. Even non-polar bonded silicas have been used with extremely high organic solvent composition, thanks to the exposed patches of silica in between the bonded ligands on the support, which can affect the interactions. [4]
The use of micelles in high performance liquid chromatography was first introduced by Armstrong and Henry in 1980. [ 2][ 3] The technique is used mainly to enhance retention and selectivity of various solutes that would otherwise be inseparable or poorly resolved. Micellar liquid chromatography (MLC) has been used in a variety of applications ...