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Considering green light around 500 nm and a NA of 1, the Abbe limit is roughly = = (0.25 μm), which is small compared to most biological cells (1 μm to 100 μm), but large compared to viruses (100 nm), proteins (10 nm) and less complex molecules (1 nm). To increase the resolution, shorter wavelengths can be used such as UV and X-ray microscopes.
The formal Rayleigh criterion is close to the empirical resolution limit found earlier by the English astronomer W. R. Dawes, who tested human observers on close binary stars of equal brightness. The result, θ = 4.56/ D , with D in inches and θ in arcseconds , is slightly narrower than calculated with the Rayleigh criterion.
Sparrow's resolution limit is nearly equivalent to the theoretical diffraction limit of resolution, the wavelength of light divided by the aperture diameter, and about 20% smaller than the Rayleigh limit. For example, in a 200 mm (eight-inch) telescope, Rayleigh's resolution limit is 0.69 arc seconds, Sparrow's resolution limit is 0.54 arc seconds.
Only the very highest quality lenses have diffraction-limited resolution, however, and normally the quality of the lens limits its ability to resolve detail. This ability is expressed by the Optical Transfer Function which describes the spatial (angular) variation of the light signal as a function of spatial (angular) frequency. When the image ...
Glasses' Abbe numbers, along with their mean refractive indices, are used in the calculation of the required refractive powers of the elements of achromatic lenses in order to cancel chromatic aberration to first order. These two parameters which enter into the equations for design of achromatic doublets are exactly what is plotted on an Abbe ...
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Gaussian beam width () as a function of the axial distance .: beam waist; : confocal parameter; : Rayleigh length; : total angular spread In optics and especially laser science, the Rayleigh length or Rayleigh range, , is the distance along the propagation direction of a beam from the waist to the place where the area of the cross section is doubled. [1]