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A kit for the purification of plasmids—small ring-shaped DNA molecules in bacterial cells. [ 5 ] 1996 saw the initial public offering of QIAGEN on the technology-oriented Nasdaq stock exchange, becoming the first German company to do so.
DNA extraction. The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. [1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other ...
Spin column-based nucleic acid purification. Silica in a spin column with water and with DNA sample in chaotropic buffer. Spin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions.
Plasmid miniprep. 0.8% agarose gel ethidium bromide -stained. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology. [1][2] Many methods have been developed to purify ...
The tag was invented by Roche, [1] although the use of histidines and its vectors are distributed by Qiagen. Various purification kits for histidine-tagged proteins are commercially available from multiple companies. [2] The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.
Lysis buffer. A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells (e.g. western blot for protein, or for DNA extraction).
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