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An action spectrum is a graph of the rate of biological effectiveness plotted against wavelength of light. [1] It is related to absorption spectrum in many systems. Mathematically, it describes the inverse quantity of light required to evoke a constant response.
This mismatch between absorption spectra and photochemical action plots has by now been observed in a wide array of photoreactive systems. [41] [42] [43] A prominent example is the photoinduced [2+2] cycloaddition of the stilbene derivative, styrypyrene, which exhibited an 80 nm discrepancy between the action plot and absorption spectrum. [33]
If the birefringence is known, then the thickness, t, of the sample can be determined; If the thickness is known, then the birefringence of the sample can be determined; As the order of the optical path difference increases, then it is more likely that more wavelengths of light will be removed from the spectrum.
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Bottom: PAR action spectrum (oxygen evolution per incident photon) of an isolated chloroplast. Chlorophyll , the most abundant plant pigment, is most efficient in capturing red and blue light. Accessory pigments such as carotenes and xanthophylls harvest some green light and pass it on to the photosynthetic process, but enough of the green ...