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Recombinase polymerase amplification (RPA) is a single tube, isothermal alternative to the polymerase chain reaction (PCR). [1] By adding a reverse transcriptase enzyme to an RPA reaction, it can detect RNA as well as DNA, without the need for a separate step to produce cDNA.
Metalink 3.0 was publicly released in 2005. It was designed to aid in downloading Linux ISO images and other large files on release day, when servers would be overloaded (each server would have to be tried manually) and to repair large downloads by replacing only the parts with errors instead of fully re-downloading them.
Because of this, tool kits that scrape web content were created. A web scraper is an API or tool to extract data from a website. [6] Companies like Amazon AWS and Google provide web scraping tools, services, and public data available free of cost to end-users. Newer forms of web scraping involve listening to data feeds from web servers.
Robotic process automation (RPA) is a form of business process automation that is based on software robots (bots) or artificial intelligence (AI) agents. [1] RPA should not be confused with artificial intelligence as it is based on automotive technology following a predefined workflow. [ 2 ]
Nuclease protection assays are used to map introns and 5' and 3' ends of transcribed gene regions. Quantitative results can be obtained regarding the amount of the target RNA present in the original cellular extract - if the target is a messenger RNA, this can indicate the level of transcription of the gene in the cell.
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At the same time, a number of new functions were announced, including robotic process automation (RPA) capabilities. [ 8 ] In 2020, Microsoft acquired Softomotive, [ 9 ] the makers of ProcessRobot and WinAutomation, to further expand the capabilities in Power Automate.
This method integrates recombinant polymerase amplification (RPA) with a vertical flow visualization strip (VF) to form an RPA-VF assay that targets the thymidine kinase gene, ensuring fast detection, high specificity, and zero cross-reactivity with other pathogens. [26] Large non-polymeric molecules