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Proteus species do not usually ferment lactose. Similar to other members of the Enterobacterales order, bacteria from the Proteus genus are glucose fermenting, oxidase-negative, catalase-positive, and nitrate-positive. Glucose fermentation in this species can be demonstrated through the triple sugar iron test.
[11] [citation needed] This clustered configuration, as well as the positive catalase test, differentiates Staphylococcus spp. from Streptococcus spp., which manifests in chains. Due to its ability to clot blood, S. pseudintermedius is subcategorized into a group of coagulase positive (CoPS) staphylococci.
A blood culture is a medical laboratory test used to detect bacteria or fungi in a person's blood. Under normal conditions, the blood does not contain microorganisms : their presence can indicate a bloodstream infection such as bacteremia or fungemia , which in severe cases may result in sepsis .
Proteus mirabilis appears as Gram-negative rods after Gram staining under bright-field microscopy with 1000 times magnification. Proteus mirabilis is a Gram-negative, facultatively anaerobic, rod-shaped bacterium. It shows swarming motility and urease activity. P. mirabilis causes 90% of all Proteus infections in humans.
Phenylethyl alcohol agar selects for Staphylococcus species while inhibiting Gram-negative bacilli (e.g., Escherichia coli, Shigella, Proteus, etc.). R2A agar, a nonspecific medium, imitates water, so is used for water analysis. Tryptic (trypticase) soy agar (TSA) is a general-purpose medium produced by enzymatic digestion of soybean meal and ...
Unlike the more commonly seen species of Proteus, P. hauseri is also able to convert tryptophan into indole, resulting in a positive indole test. P. hauser i shares a similar biochemical profile with Proteus vulgaris but can be differentiated by its ability to produce acid from trehalose.
Hemolyses of Streptococcus spp. (left) α-hemolysis (S. mitis); (middle) β-hemolysis (S. pyogenes); (right) γ-hemolysis (non-hemolytic, S. salivarius) Hemolysis is the breakdown of red blood cells. The ability of bacterial colonies to induce hemolysis when grown on blood agar is used to classify certain microorganisms.
The organism on the right produced no color, so it does not appear to be a lactose fermenter. MacConkey agar is a selective and differential culture medium for bacteria . It is designed to selectively isolate gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation . [ 1 ]