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Proteus species do not usually ferment lactose. Similar to other members of the Enterobacterales order, bacteria from the Proteus genus are glucose fermenting, oxidase-negative, catalase-positive, and nitrate-positive. Glucose fermentation in this species can be demonstrated through the triple sugar iron test.
Hemolyses of Streptococcus spp. (left) α-hemolysis (S. mitis); (middle) β-hemolysis (S. pyogenes); (right) γ-hemolysis (non-hemolytic, S. salivarius) Hemolysis is the breakdown of red blood cells. The ability of bacterial colonies to induce hemolysis when grown on blood agar is used to classify certain microorganisms.
The Proteus penneri group of bacteria was named in 1982. It reclassified a group of strains formerly known as Proteus vulgaris biogroup 1. [6] In 1978, Brenner et al. showed through DNA hybridization studies that P. vulgaris was a heterogenous species. [7]
The swarming capability of Proteus mirabilis is important because it is implicated in the pathogenesis of the bacteria and the swarming capability is associated with the bacteria's ability to express virulence factors [9] Proteus mirabilis has a very characteristic bulls-eye appearance on an agar plate due to the regular periodic cycling ...
Thayer–Martin agar (or Thayer–Martin medium, or VPN agar) is a Mueller–Hinton agar with 5% chocolate sheep blood and antibiotics. It is used for culturing and primarily isolating pathogenic Neisseria bacteria , including Neisseria gonorrhoeae and Neisseria meningitidis , as the medium inhibits the growth of most other microorganisms.
Colonial morphology serves as the first step in the identification of microbial species from clinical samples. [10] Based on the visual appearance of the colonies, microbiologists can narrow down the list of possible organisms, allowing them to select appropriate tests to provide a definitive diagnosis.
Salmonella spp appear to be yellow or colourless colonies, often with a dark centre. As there are many bacteria that also look like Salmonella on DCA, it is widely recommended that more selective agars are used for the identification of Salmonella, namely xylose lysine deoxycholate (XLD) agar. This growth medium is heat-sensitive and should be ...
Where available, the need to identify immune reactors can be avoided by first inoculating the sample onto differential agar like Staphylococcus Medium 110, [19] which inhibit the growth of non-staphylococcal bacteria. Cytology alone does not allow for the differentiation between different species in the Staphylococcus genus.