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Tris is also used as a primary standard to standardize acid solutions for chemical analysis. Tris is used to increase permeability of cell membranes. [ 13 ] It is a component of the Moderna COVID-19 vaccine [ 14 ] and the Pfizer-BioNTech COVID-19 vaccine for use in children 5 through 11 years of age.
The conjugate base of NTA is a tripodal tetradentate trianionic ligand, forming coordination compounds with a variety of metal ions. [ 8 ] Like EDTA , its sodium salt is used for water softening to remove Ca 2+ .
Tris-buffered saline (TBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Tris (with HCl) has a slightly alkaline buffering capacity in the 7–9.2 range. The conjugate acid of Tris has a pK a of 8.07 at 25 °C.
TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. In molecular biology, it is used in agarose electrophoresis typically for the separation of nucleic acids such as DNA and RNA. [1] It is made up of Tris-acetate buffer, usually at pH 8.3, and EDTA, which sequesters divalent cations.
10 mM Tris, bring to pH 8.0 with HCl; 1 mM EDTA, bring to pH 8.0 with NaOH; TE buffer is also known as T 10 E 1 buffer, which can be read as "T ten E one buffer". To make a 100 ml solution of T 10 E 1 buffer, 1 ml of 1 M Tris base (pH 10–11) and 0.2 ml EDTA (0.5 M) are mixed and made up with double distilled water up to 100ml. Add microliter ...
54 g of Tris base (CAS# 77-86-1, free base) 27.5 g of boric acid (CAS# 10043-35-3) 20 ml of 0.5 M EDTA (CAS# 60-00-4) (pH 8.0) Adjust pH to 8.3 by HCl. [2] TBE can be diluted to 1X prior to use in electrophoresis, 0.5x is acceptable as well. Higher concentrations will result in poor results due to excessive heat generation.
TSE or Tris/Saline/EDTA, is a buffer solution containing a mixture of Tris base, Sodium chloride and EDTA. In molecular biology, TSE buffers are often used in procedures involving nucleic acids . Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water.
Tris(1,3-dichloroisopropyl)phosphate (TDCPP) is a chlorinated organophosphate. Organophosphate chemicals have a wide variety of applications and are used as flame retardants , pesticides , plasticizers , and nerve gases .