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REBASE contains an extensive set of references, sites of recognition and cleavage, sequences and structures. It also contains information on the commercial availability of each enzyme. REBASE is one of the longest running biological databases having its roots in a collection of restriction enzymes maintained by Richard J. Roberts since before ...
New England Biolabs (NEB) is an American life sciences company which produces and supplies recombinant and native enzyme reagents for life science research. [2] It also provides products and services supporting genome editing , synthetic biology and next-generation sequencing . [ 3 ]
In molecular biology, XhoI is a type II restriction enzyme EC that recognise the double-stranded DNA sequence CTCGAG and cleaves after C-1. [1] Type II restriction endonucleases are components of prokaryotic DNA restriction-modification mechanisms that protect the organism against invading foreign DNA.
Name of Restriction Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature, [1] [2] and bibliographical references. Note: When alphabetizing, enzymes are first ordered alphabetically by the acronyms (everything before the roman numeral); then enzymes of a given acronym are ordered alphabetically by the ...
A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. [1] [2] [3] Restriction enzymes are one class of the broader endonuclease group of enzymes.
It combines restriction enzymes and bisulfite sequencing to enrich for areas of the genome with a high CpG content. Due to the high cost and depth of sequencing to analyze methylation status in the entire genome, Meissner et al. developed this technique in 2005 to reduce the amount of nucleotides required to sequence to 1% of the genome. [1]
Oligomer Restriction (abbreviated OR) is a procedure to detect an altered DNA sequence in a genome. A labeled oligonucleotide probe is hybridized to a target DNA, and then treated with a restriction enzyme. If the probe exactly matches the target, the restriction enzyme will cleave the probe, changing its size.
A rare-cutter enzyme is a restriction enzyme with a recognition sequence which occurs only rarely in a genome. An example is NotI, which cuts after the first GC of a 5'-GCGGCCGC-3' sequence; restriction enzymes with seven and eight base pair recognition sequences are often also called rare-cutter enzymes (six bp recognition sequences are much more common).