Search results
Results From The WOW.Com Content Network
Original file (2,000 × 1,125 pixels, file size: 12 KB, MIME type: application/pdf) This is a file from the Wikimedia Commons . Information from its description page there is shown below.
Many site-specific recombination systems have been identified to perform these DNA rearrangements for a variety of purposes, but nearly all of these belong to either of two families, tyrosine recombinases (YR) and serine recombinases (SR), depending on their mechanism. These two families can mediate up to three types of DNA rearrangements ...
Download QR code; In other projects ... File:DNA polymerization.pdf → File:DNA condensation.svg. ... Page size: 612 x 792 pts (letter) Version of PDF format: 1.3
The RPA process employs three core enzymes – a recombinase, a single-stranded DNA-binding protein (SSB) and strand-displacing polymerase. Recombinases are capable of pairing oligonucleotide primers with homologous sequence in duplex DNA. [1] SSB bind to displaced strands of DNA and prevent the primers from being displaced.
DNA shuffling is useful for generating proteins with novel properties or combinations of desired properties. [1] DNA shuffling, also known as molecular breeding, is an in vitro random recombination method to generate mutant genes for directed evolution and to enable a rapid increase in DNA library size.
NCO recombinants are thought to occur primarily by the Synthesis Dependent Strand Annealing (SDSA) model, illustrated on the left, above. Most recombination events appear to be the SDSA type. Meiotic recombination may be initiated by double-stranded breaks that are introduced into the DNA by exposure to DNA damaging agents, [9] or the Spo11 ...
Gibson assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single, isothermal reaction. It is named after its creator, Daniel G. Gibson, who is the chief technology officer and co-founder of the synthetic biology company, Telesis Bio.
The enzyme (38 kDa) is a member of the integrase family of site specific recombinase and it is known to catalyse the site specific recombination event between two DNA recognition sites . This 34 base pair (bp) loxP recognition site consists of two 13 bp palindromic sequences which flank an 8bp spacer region.