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  2. Bisulfite sequencing - Wikipedia

    en.wikipedia.org/wiki/Bisulfite_sequencing

    Bisulfite [1] sequencing (also known as bisulphite sequencing) is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied.

  3. Whole genome bisulfite sequencing - Wikipedia

    en.wikipedia.org/wiki/Whole_genome_bisulfite...

    [7] [5] Since its development, many various protocols of whole genome bisulfite sequencing have been developed aiming to improve the efficiency and efficacy of its single-base mapping. As the costs of next-generation sequencing have decreased, whole genome bisulfite sequencing has become more widely used in clinical and experimental research. [3]

  4. DNA methylation - Wikipedia

    en.wikipedia.org/wiki/DNA_methylation

    Whole genome bisulfite sequencing, also known as BS-Seq, which is a high-throughput genome-wide analysis of DNA methylation. It is based on the aforementioned sodium bisulfite conversion of genomic DNA, which is then sequenced on a Next-generation sequencing platform. The sequences obtained are then re-aligned to the reference genome to ...

  5. Combined bisulfite restriction analysis - Wikipedia

    en.wikipedia.org/wiki/Combined_Bisulfite...

    The first few steps of COBRA, and the molecular changes caused by each step to methylated and unmethylated CpG sites. Combined Bisulfite Restriction Analysis (or COBRA) is a molecular biology technique that allows for the sensitive quantification of DNA methylation levels at a specific genomic locus on a DNA sequence in a small sample of genomic DNA. [1]

  6. Bisulfite - Wikipedia

    en.wikipedia.org/wiki/Bisulfite

    Bisulfite is also the key ingredient in the Bucherer reaction. In this reaction an aromatic hydroxyl group is converted to the corresponding amine group. This is a reversible reaction. The first step in this reaction is an addition reaction of sodium bisulfite to an aromatic double bond.

  7. Reduced representation bisulfite sequencing - Wikipedia

    en.wikipedia.org/wiki/Reduced_representation...

    Reduced representation bisulfite sequencing (RRBS) is an efficient and high-throughput technique for analyzing the genome-wide methylation profiles on a single nucleotide level. It combines restriction enzymes and bisulfite sequencing to enrich for areas of the genome with a high CpG content.

  8. Single cell epigenomics - Wikipedia

    en.wikipedia.org/wiki/Single_cell_epigenomics

    This is similar to single cell genome sequencing, but with the addition of a bisulfite treatment before sequencing. Forms include whole genome bisulfite sequencing, [4] [5] and reduced representation bisulfite sequencing [6] [7] Comparison of single cell DNA methylation sequencing methods in terms of coverage as at 2015 on Mus musculus

  9. Tet methylcytosine dioxygenase 1 - Wikipedia

    en.wikipedia.org/wiki/Tet_methylcytosine_di...

    Ten-eleven translocation methylcytosine dioxygenase 1 (TET1) is a member of the TET family of enzymes, in humans it is encoded by the TET1 gene.Its function, regulation, and utilizable pathways remain a matter of current research while it seems to be involved in DNA demethylation and therefore gene regulation, [5] [6] but is expressed as different isoforms which may have distinct functions.