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High Resolution Melt (HRM) analysis is a powerful technique in molecular biology for the detection of mutations, polymorphisms and epigenetic differences in double-stranded DNA samples. It was discovered and developed by Idaho Technology and the University of Utah. [1] It has advantages over other genotyping technologies, namely:
One major difference between DNA and RNA is the sugar, ... ssDNA vs. dsDNA. ... acting as part of the restriction modification system. [129] In technology, ...
Restriction endonucleases may be found that cleave standard dsDNA (double-stranded DNA), or ssDNA (single-stranded DNA), or even RNA. [citation needed] This discussion is restricted to dsDNA; however, the discussion can be extended to the following: Standard dsDNA; Non-standard DNA; Holliday junctions
Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature (T m) is defined as the temperature at which half of the DNA strands are in the random coil or single-stranded (ssDNA) state. T m depends on the length of the DNA molecule and its specific ...
Recombineering with ssDNA provided a breakthrough both in the efficiency of the reaction and the ease of making point mutations. [1] This technique was further enhanced by the discovery that by avoiding the methyl-directed mismatch repair system, the frequency of obtaining recombinants can be increased to over 10 7 /10 8 viable cells. [14]
DNA end resection, also called 5′–3′ degradation, is a biochemical process where the blunt end of a section of double-stranded DNA (dsDNA) is modified by cutting away some nucleotides from the 5' end to produce a 3' single-stranded sequence.
Orthopoxvirus particles. A DNA virus is a virus that has a genome made of deoxyribonucleic acid (DNA) that is replicated by a DNA polymerase.They can be divided between those that have two strands of DNA in their genome, called double-stranded DNA (dsDNA) viruses, and those that have one strand of DNA in their genome, called single-stranded DNA (ssDNA) viruses. dsDNA viruses primarily belong ...
On the left is a drawing of the complex formed between alpha-hemolysin and dsDNA with linkage through an oligomer.On the right, movement of this complex in relation to a nanopore channel is shown sequentially in two steps (I) and (II).