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Historically a cesium chloride (CsCl) solution was often used, but more commonly used density gradients are sucrose or Percoll.This application requires a solution with high density and yet relatively low viscosity, and CsCl suits it because of its high solubility in water, high density owing to the large mass of Cs, as well as low viscosity and high stability of CsCl solutions.
The caesium chloride structure adopts a primitive cubic lattice with a two-atom basis, where both atoms have eightfold coordination. The chloride atoms lie upon the lattice points at the corners of the cube, while the caesium atoms lie in the holes in the center of the cubes; an alternative and exactly equivalent 'setting' has the caesium ions at the corners and the chloride ion in the center.
Differential centrifugation is the simplest method of fractionation by centrifugation, [9] commonly used to separate organelles and membranes found in cells. Organelles generally differ from each other in density and in size, making the use of differential centrifugation, and centrifugation in general, possible.
Differential centrifugation, on the other hand, does not utilize a density gradient, and the centrifugation is taken in increasing speeds. The different centrifugation speeds often create separation into not more than two fractions, so the supernatant can be separated further in additional centrifugation steps.
Large centrifuges are used to simulate high gravity or acceleration environments (for example, high-G training for test pilots). Medium-sized centrifuges are used in washing machines and at some swimming pools to draw water out of fabrics. Gas centrifuges are used for isotope separation, such as to enrich nuclear fuel for fissile isotopes.
Pumps being used to dewater a spillway at Baldhill Dam. Dewatering / d iː ˈ w ɔː t ər ɪ ŋ / is the removal of water from a location. This may be done by wet classification, centrifugation, filtration, or similar solid-liquid separation processes, such as removal of residual liquid from a filter cake by a filter press as part of various industrial processes.
Elutriation is a common method used by biologists to sample meiofauna. [4] The sediment sample is constantly agitated by a flow of filtered water from below, the action of which dislodges interstitial organisms embedded between sediment grains. A very fine filter at the top captures these organisms from the overflow. [5]
Diagram of a gas centrifuge with countercurrent flow, used for separating isotopes of uranium. A gas centrifuge is a device that performs isotope separation of gases. A centrifuge relies on the principles of centrifugal force accelerating molecules so that particles of different masses are physically separated in a gradient along the radius of a rotating container.