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The C Programming Language (sometimes termed K&R, after its authors' initials) is a computer programming book written by Brian Kernighan and Dennis Ritchie, the latter of whom originally designed and implemented the C programming language, as well as co-designed the Unix operating system with which development of the language was closely intertwined.
Controlled-rate and slow freezing, also known as slow programmable freezing (SPF), [18] is a technique where cells are cooled to around -196 °C over the course of several hours. Slow programmable freezing was developed during the early 1970s, and eventually resulted in the first human frozen embryo birth in 1984. Since then, machines that ...
The ultimate objective is to freeze the specimen so rapidly (at 10 4 to 10 6 K per second) that ice crystals are unable to form, or are prevented from growing big enough to cause damage to the specimen's ultrastructure. The formation of samples containing specimens in amorphous ice is the "holy grail" of biological cryomicroscopy. [citation needed]
With cryoablation, areas of tissue can be mapped by limited, reversible, freezing (e.g., to -10 C). If the result is undesirable, the tissue can be rewarmed without permanent damage. Otherwise, the tissue can be permanently ablated by freezing it to a lower temperature (e.g., -73 C).
For red blood cells, the optimum cooling rate is very rapid (nearly 100 °C per second), whereas for stem cells the optimum cooling rate is very slow (1 °C per minute). Cryoprotectants, such as dimethyl sulfoxide and glycerol, are used to protect cells from freezing. A variety of cell types are protected by 10% dimethyl sulfoxide. [18]
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This is an accepted version of this page This is the latest accepted revision, reviewed on 10 January 2025. General-purpose programming language "C programming language" redirects here. For the book, see The C Programming Language. Not to be confused with C++ or C#. C Logotype used on the cover of the first edition of The C Programming Language Paradigm Multi-paradigm: imperative (procedural ...
The United States Food and Drug Administration issued multiple advisories on Wednesday for oysters and clams that they say could be contaminated with norovirus, a serious gastrointestinal illness ...