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It is ideally spatially unstructured and temporally unstructured, in a steady state defined by the rates of nutrient supply and bacterial growth. In comparison to batch culture, bacteria are maintained in exponential growth phase, and the growth rate of the bacteria is known. Related devices include turbidostats and auxostats.
The Monod equation is a mathematical model for the growth of microorganisms. It is named for Jacques Monod (1910–1976, a French biochemist, Nobel Prize in Physiology or Medicine in 1965), who proposed using an equation of this form to relate microbial growth rates in an aqueous environment to the concentration of a limiting nutrient.
The rate of nutrient exchange is expressed as the dilution rate D. At steady state, the specific growth rate μ of the micro-organism is equal to the dilution rate D. The dilution rate is defined as the flow of medium per unit of time, F, over the volume V of culture in the bioreactor
Figure 1: A bi-phasic bacterial growth curve.. A growth curve is an empirical model of the evolution of a quantity over time. Growth curves are widely used in biology for quantities such as population size or biomass (in population ecology and demography, for population growth analysis), individual body height or biomass (in physiology, for growth analysis of individuals).
The plate reader is set to read optical density at 650 nm every 5 minutes for 12 hours, shaking before each reading. Raw data is imported into Microsoft Excel, where the macro VCC Calculate is run to determine the time required for each growth curve to reach a threshold optical density of 0.02.
As resources become more limited, the growth rate tapers off, and eventually, once growth rates are at the carrying capacity of the environment, the population size will taper off. [6] This S-shaped curve observed in logistic growth is a more accurate model than exponential growth for observing real-life population growth of organisms. [8]
The total number of living aerobic bacteria can be determined using a plate count agar which is a substrate for bacteria to grow on. The medium contains casein which provides nitrogen, carbon, amino acids, vitamins and minerals to aid in the growth of the organism. Yeast extract is the source for vitamins, particularly of B-group. Glucose is ...
The log phase is marked by rapid exponential growth. The rate at which cells grow during this phase is known as the growth rate (k), and the time it takes the cells to double is known as the generation time (g). During log phase, nutrients are metabolised at maximum speed until one of the nutrients is depleted and starts limiting growth.