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The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]
In addition, finding the appropriate antibody could require extensive screening of many antibodies by western blotting prior to beginning RPMA analysis. [1] To overcome this issue, two open resource databases have been created to display western blot results for antibodies that have good binding specificity within the expected range.
[6] [15] This can improve precision (down to 0.1 μg of total protein per lane), cost-effectiveness, and data reliability. [16] Fluorescent stains and stain-free gels require special equipment to visualize the proteins on the gel/blot. [5] Stains may not cover the blot evenly; more stain might collect towards the edges of the blot than in the ...
The southwestern blot, is a lab technique that involves identifying as well as characterizing DNA-binding proteins [1] by their ability to bind to specific oligonucleotide probes. Determination of molecular weight of proteins binding to DNA is also made possible by the technique.
Each of the approaches has its own strengths and weaknesses, especially with regard to the sensitivity and specificity of the method. [1] A high sensitivity means that many of the interactions that occur are detected by the screen. A high specificity indicates that most of the interactions detected by the screen are occurring in reality.
Gel zymography is often used for the detection and analysis of enzymes produced by microorganisms. [7] This has led to variations on the standard protocol e.g. mixed-substrate zymography. [2] Reverse zymography copolymerizes both the substrate and the enzyme with the acrylamide, and is useful for the demonstration of enzyme inhibitor activity ...
A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. [1]
The far-western blot, or far-western blotting, is a molecular biological method based on the technique of western blot to detect protein-protein interaction in vitro. [1] [2] Whereas western blot uses an antibody probe to detect a protein of interest, far-western blot uses a non-antibody probe which can bind the protein of interest. Thus ...