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The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample. From here, the plates are rotated to ensure the samples are uniformly mixing with the agar. Incubation of the plates is the next step and is carried out for about 3 days at 20 to 30 degrees Celsius.
Contamination on an agar plate. An agar plate is a Petri dish that contains a growth medium solidified with agar, used to culture microorganisms. Sometimes selective compounds are added to influence growth, such as antibiotics. [1] 96 pinner used to perform spot assays with yeast, fungal or bacterial cells
Agar plates Organisms are placed or streaked onto petri dishes Provides a solid surface for stationary growth, compact and stackable Agar based dipsticks Essentially miniature agar plates in the form of dipsticks Diagnostic purposes, can be used anywhere, cost effective, easy to use Selective and differential media
The Petri dish was developed by German physician Julius Richard Petri (after whom the name is given) while working as an assistant to Robert Koch at Berlin University.Petri did not invent the culture dish himself; rather, it was a modified version of Koch's invention [9] which used an agar medium that was developed by Walther Hesse. [10]
Microtitre plates: mostly used for ELISA: Microtome: cuts prepared specimens for analysis under microscope Nichrome wire loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc.; sterilized by flaming to red hot before use Petri dish/agar plate: to act as a supporting container to hold the ...
Cell spreaders. In microbiology, a cell spreader or plate spreader is a tool used to smoothly spread cells and bacteria on a culture plate, such as a petri dish.. Cell spreaders can be made from glass, plastic, or metal, and come in various shapes.
Glass evaporating dishes, such as watch glasses, are primarily used as an evaporating surface (though they may be used to cover a beaker.) The Petri dish is a flat dish filled with a nutritious gelatin that allows for microorganisms to quickly grow, its named after its inventor Julius Petri in the 1880s.
In microbiology, a culture plate is a low flat-bottomed laboratory container for growing a layer of organisms such as bacteria, molds, and cells on a thin layer of nutrient medium. The most common types are the petri dish [ 1 ] [ 2 ] and multiwell plates .