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While at Merck & Co., where he was hired as a researcher in 1950, he led a team that developed a method to synthesize the enzyme ribonuclease.His team was successful, with their results announced in January 1969 parallel with those from a separate team led by Bernd Gutte and Robert Bruce Merrifield at Rockefeller University who also achieved synthesis of the same enzyme using a different method.
The evolutionary motivation for a decoupled, two-step nitrification reaction is an area of ongoing research. In 2015, it was discovered that the species Nitrospira inopinata possesses all the enzymes required for carrying out complete nitrification in one step, suggesting that this reaction does occur. [12] [13]
Enzyme kinetics and mechanism: Awards: Alexander von Humboldt Senior Scientist Award, Repligen Corporation Award in Chemistry of Biological Processes: Scientific career: Fields: Biochemistry: Institutions: Harvard University, Ohio State University, University of Wisconsin-Madison: Doctoral advisor: Robert H. Abeles
Arthur Kornberg (March 3, 1918 – October 26, 2007) was an American biochemist who won the Nobel Prize in Physiology or Medicine 1959 for the discovery of "the mechanisms in the biological synthesis of ribonucleic acid and deoxyribonucleic acid" together with Spanish biochemist and physician Severo Ochoa of New York University.
A much larger number of recombinant proteins is used in the research laboratory. These include both commercially available proteins (for example most of the enzymes used in the molecular biology laboratory), and those that are generated in the course specific research projects.
After earning his Ph.D., Dr. Lardy joined the faculty at the Enzyme Institute at UW-Madison, and very quickly became the institute's team lead. [6] His laboratory has published more than 370 articles on a variety of metabolic phenomena. Even after his official retirement, Prof. Lardy still operated a laboratory in the biochemistry department. [3]
David Ezra Green (August 5, 1910 – July 8, 1983) was an American biochemist who made significant contributions to the study of enzymes, particularly the electron transport chain and oxidative phosphorylation. [1]
Genes coding for the enzyme are primarily expressed in the liver, in the kidney cortex and (to a lesser extent) in the β-cells of the pancreatic islets and intestinal mucosa (especially during times of starvation). [7] Glucose 6-phosphatase is present in a wide variety of muscles across the animal kingdom, albeit at very low concentrations. [10]