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ISO 10936 Optics and photonics – Operation microscopes ISO 10936-1:2017 Part 1: Requirements and test methods; ISO 10936-2:2010 Part 2: Light hazard from operation microscopes used in ocular surgery; ISO 10938:2016 Ophthalmic optics – Chart displays for visual acuity measurement – Printed, projected and electronic; ISO 10939:2017 ...
The lateral resolution of light sheet fluorescence microscopy can be improved beyond the Abbe limit, by using super resolution microscopy techniques, e.g. with using the fact, that single fluorophores can be located with much higher spatial precision than the nominal resolution of the used optical system (see stochastic localization microscopy ...
Additional flexibility can be added by using digital light-sheet microscopy to generate the illumination patterns. In digital LSM, the light sheet is created by rapidly scanning a laser beam through the sample. [3] [2] [14] This allows for fine control over the specific illumination pattern by modulating the intensity of the laser as it scans ...
Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can ...
Polarized light microscopy can mean any of a number of optical microscopy techniques involving polarized light. Simple techniques include illumination of the sample with polarized light. Directly transmitted light can, optionally, be blocked with a polariser oriented at 90 degrees to the illumination. More complex microscopy techniques which ...
Diagram illustrating near-field optics, with the diffraction of light coming from NSOM fiber probe, showing wavelength of light and the near-field. [1] Comparison of photoluminescence maps recorded from a molybdenum disulfide flake using NSOM with a campanile probe (top) and conventional confocal microscopy (bottom).
Photoemission electron microscopy (PEEM, also called photoelectron microscopy, PEM) is a type of electron microscopy that utilizes local variations in electron emission to generate image contrast. [ citation needed ] The excitation is usually produced by ultraviolet light , synchrotron radiation or X-ray sources.
Fiji is most popular in the life sciences community, where the 3D Viewer [12] helps visualizing data obtained through light microscopy, and for which Fiji provides registration, [13] segmentation, and other advanced image processing algorithms.