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Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue.
Gram stain of Candida albicans from a vaginal swab. The small oval chlamydospores are 2–4 μm in diameter. A photomicrograph of Candida albicans showing hyphal outgrowth and other morphological characteristics. Some species of yeast are opportunistic pathogens that can cause infection in people with compromised immune systems.
The pink blobs are vaginal epithelial cells and the dark granules are common artefacts of the Gram-stain. (Note: microscope lenses do not have F-numbers they have Numerical apertures, this one was NA 1.25 and oil immersion). Articles in which this image appears Candidiasis, Candida albicans, Yeast, Gram stain, Chlamydospore, Fungus
The Gram stain, developed in 1884 by Hans Christian Gram, characterises bacteria based on the structural characteristics of their cell walls. [177] [77] The thick layers of peptidoglycan in the "Gram-positive" cell wall stain purple, while the thin "Gram-negative" cell wall appears pink. [177]
A small intestine sample stained using the Grocott's methenamine silver stain demonstrating histoplasma (black round yeast with narrow budding) in a granuloma. In pathology, the Grocott–Gömöri's methenamine silver stain, abbreviated GMS, is a popular staining method in histology.
Hematology: Blood smears on a glass slide, stained and ready to be examined under the microscope. Bacteriology: Agar plate with bacterial colonies. Bacteriology: microscopic image of a mixture of two types of bacteria stained with the Gram stain. Clinical chemistry: an automated blood chemistry analyser.
Under the microscope, the India ink stain is used for easy visualization of the capsule in cerebral spinal fluid. [10] The particles of ink pigment do not enter the capsule that surrounds the spherical yeast cell, resulting in a zone of clearance or "halo" around the cells. This allows for quick and easy identification of C. neoformans.