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The CMS Consensus-Based Equity quality measure incorporates best practices on blood culture collection. Find tips and review relevant professional guidance and Clinical Laboratory Improvement Amendments of 1988 (CLIA) regulations to help ensure optimal blood culture collection.
This document provides recommendations for the collection, transport, and processing of blood cultures as well as guidance for the recovery of pathogens from blood specimens taken from patients who are suspected of having bacteremia or fungemia.
Blood is a sterile fluid, and a normal, healthy patient does not have bacteria in the blood. However, many bacteria do colonize the skin. It is therefore most important to use proper, sterile technique to decontaminate the patient’s skin before collecting the blood culture.
• The preferred method for obtaining blood cultures is by peripheral venipuncture. These have the lowest rate of false positive cultures. • Strict aseptic technique and best practice should be used to obtain blood cultures. (see Phlebotomy Services, Blood Culture Collection policy and Nursing Policy, Blood Cultures for protocol)
offer an illustrated step-by-step guide to best blood culture collection practices. This booklet is intended to be a useful reference tool for physicians, nurses, clinical microbiologists, phlebotomists, laboratory personnel and all other healthcare professionals involved in the blood culture process. * AST: Antimicrobial Susceptibility Testing
The document highlights the medical indications for blood cultures along with standardized processes for specimen collection as well as methods to optimize recovery of pathogens. The guideline also presents up to date information about the use of blood cultures for special patient populations, as these populations present special challenges for ...
• Use a Mycobacterial blood culture bottle (BD BACTEC Myco/F Lytic culture bottle). Because the media is unstable, bottles must be obtained from the Microbiology Lab (5-6510 option #1).
Recommended volume: 1 to 2 cc. of blood per bottle. Blood Culture for TB: two heparin (green top) or two SPS (yellow top) Vacutainers. No more than once per 24 hour period. Blood Culture for Fungus: One aerobic (blue top) blood culture bottle.
Principles and Procedures for Blood Cultures. This guideline includes recommendations for collecting, transporting, and processing specimens for blood culture, as . well as procedures for recovering pathogens from the blood of . patients with suspected bacteremia or fungemia.
These recommendations illustrate the best practices for blood culture collection based on the World Health Organization recommendations (WHO guidelines on drawing blood: best practices in phlebotomy. 2010. ISBN 978 92 4 159922 1). Best practices may vary between healthcare facilities; refer to guidelines applicable in your facility.