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The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
Compound microscopes first appeared in Europe around 1620. [2] [3] The actual inventor of the compound microscope is unknown although many claims have been made over the years. These include a dubious claim that Dutch spectacle-maker Zacharias Janssen invented the compound microscope and the telescope as early as 1590.
Description: Binocular compound microscope from 1914; Carl Zeiss (1816–88), Jena, Germany; materials: brass, metal, glass; owner: The Golub Collection, University ...
Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...
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The focus of the first lens is traditionally about 2mm away from the plane face coinciding with the sample plane. A pinhole cap can be used to align the optical axis of the condenser with that of the microscope. The Abbe condenser is still the basis for most modern light microscope condenser designs, even though its optical performance is poor.
The success of the phase-contrast microscope has led to a number of subsequent phase-imaging methods. In 1952, Georges Nomarski patented what is today known as differential interference contrast (DIC) microscopy. [8] It enhances contrast by creating artificial shadows, as if the object is illuminated from the side.
In contrast to binoculars, it allows partially stereoscopic viewing and partially monocular viewing, this because the eyes and brain still process the image binocularly, as both images are produced by the same objective and do not differ except for aberrations induced by the binoviewer itself.