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Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy , imaging probes, and spectroscopy .
Endomicroscopy is a technique for obtaining histology-like images from inside the human body in real-time, [1] [2] [3] a process known as ‘optical biopsy’. [4] [5] It generally refers to fluorescence confocal microscopy, although multi-photon microscopy and optical coherence tomography have also been adapted for endoscopic use.
Molecular imaging with antibodies may be applied to CLE as a diagnostic benchmark due to high correlation with ex vivo microscopy. [21] The molecular imaging technique can be used in a similar manner in the examination of head and neck cancer using CLE, though the diagnostic targets may be different from those in the gastrointestinal tract. [20 ...
The paper used three-photon fluorescence microscopy at a spectral excitation window of 1,320 nm to imaging the mouse brain structure and function through the intact skull with high spatial and temporal resolution(The lateral and axial FWHM was 0.96μm and 4.6μm) and large FOVs (hundreds of micrometers), and at substantial depth(>500 μm). This ...
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century.
High quality of modern microscopes and imaging software also permits subcellular imaging in live animals that in turn allows studying cell biology at molecular level in vivo. Advancements in fluorescent protein technology and genetic tools that enable controlled expression of a given gene at a specific time in a tissue of interest also played ...
This is not practical in intact animals, and in addition, the temporal resolution of bioluminescence imaging is relatively poor (seconds-minutes). The first genetically encoded fluorescent calcium indicator (GECI) to be used to image activity in an animal was cameleon, designed by Atsushi Miyawaki, Roger Tsien and coworkers in 1997. [1]
Additionally, fiber photometry can be coupled with in vivo electrophysiology within the same animal. [33] When combined, this combination of electrophysiological recording and calcium imaging can be used to observe cell-type specific activity with higher temporal precision read-outs of neuronal action potentials in freely-behaving animal models.