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Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy , imaging probes, and spectroscopy .
High quality of modern microscopes and imaging software also permits subcellular imaging in live animals that in turn allows studying cell biology at molecular level in vivo. Advancements in fluorescent protein technology and genetic tools that enable controlled expression of a given gene at a specific time in a tissue of interest also played ...
The imaging procedure is more complex for FRET sensors, but the fluorescence ratio can be calibrated to measure the absolute concentration of a ligand. Read-out via fluorescence lifetime imaging (FLIM) of donor fluorescence is also possible, as the FRET process speeds up the fluorescence decay.
Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.
The paper used three-photon fluorescence microscopy at a spectral excitation window of 1,320 nm to imaging the mouse brain structure and function through the intact skull with high spatial and temporal resolution(The lateral and axial FWHM was 0.96μm and 4.6μm) and large FOVs (hundreds of micrometers), and at substantial depth(>500 μm). This ...
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century.
The first uses of FGS dates back to the 1940s when fluorescein was first used in humans to enhance the imaging of brain tumors, cysts, edema and blood flow in vivo. [15] In modern times the use has fallen off, until a multicenter trial in Germany concluded that FGS to help guide glioma resection based upon fluorescence from PpIX provided ...
In a live single-cell imaging experiment a fluorescent reporter is introduced into a cell line to measure the levels, localisation or activity of a signalling molecule. Subsequently, a population of cells is imaged over time with careful atmospheric control to maintain viability, and reduce stress upon the cells.