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The main advantage of intravital microscopy is that it allows imaging living cells while they are in the true environment of a complex multicellular organism. Thus, intravital microscopy allows researchers to study the behavior of cells in their natural environment or in vivo rather than in a cell culture. Another advantage of intravital ...
[20] [21] Quantitative phase-contrast microscopy has an advantage over fluorescent and phase-contrast microscopy in that it is both non-invasive and quantitative in its nature. Due to the narrow focal depth of conventional microscopy, live-cell imaging is to a large extent currently limited to observing cells on a single plane.
Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.
Molecular imaging with antibodies may be applied to CLE as a diagnostic benchmark due to high correlation with ex vivo microscopy. [21] The molecular imaging technique can be used in a similar manner in the examination of head and neck cancer using CLE, though the diagnostic targets may be different from those in the gastrointestinal tract. [20 ...
A number of near-infrared (NIR) fluorophores have been employed for in vivo imaging, including Kodak X-SIGHT Dyes and Conjugates, Pz 247, DyLight 750 and 800 Fluors, Cy 5.5 and 7 Fluors, Alexa Fluor 680 and 750 Dyes, IRDye 680 and 800CW Fluors. Quantum dots, with their photostability and bright emissions, have generated a great deal of interest ...
In systems biology, live single-cell imaging is a live-cell imaging technique that combines traditional live-cell imaging and time-lapse microscopy techniques with automated cell tracking and feature extraction, drawing many techniques from high-content screening. It is used to study signalling dynamics and behaviour in populations of ...
These advantages of three-photon microscopy gives it an edge in visualize in vivo and ex vivo tissue morphology and physiology at a cellular level deep within scattering tissue [4] and Rapid volumetric imaging. [17] In the recent study, Xu has demonstrated the potential of three-photon imaging for noninvasive studies of live biological systems ...
This is a laboratory rat with a brain implant, that was used to record in vivo neuronal activity. Studies that are in vivo (Latin for "within the living"; often not italicized in English [1] [2] [3]) are those in which the effects of various biological entities are tested on whole, living organisms or cells, usually animals, including humans, and plants, as opposed to a tissue extract or dead ...