Search results
Results From The WOW.Com Content Network
HPLC is widely used for manufacturing (e.g., during the production process of pharmaceutical and biological products), [4] [5] legal (e.g., detecting performance enhancement drugs in urine), [6] research (e.g., separating the components of a complex biological sample, or of similar synthetic chemicals from each other), and medical (e.g ...
An assay (analysis) is never an isolated process, as it must be accompanied with pre- and post-analytic procedures. Both the communication order (the request to perform an assay plus related information) and the handling of the specimen itself (the collecting, documenting, transporting, and processing done before beginning the assay) are pre-analytic steps.
The charged aerosol detector (CAD) is a detector used in conjunction with high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) to measure the amount of chemicals in a sample by creating charged aerosol particles which are detected using an electrometer.
The use of trapezoidal rule in AUC calculation was known in literature by no later than 1975, in J.G. Wagner's Fundamentals of Clinical Pharmacokinetics. A 1977 article compares the "classical" trapezoidal method to a number of methods that take into account the typical shape of the concentration plot, caused by first-order kinetics. [8]
Standard addition involves adding known amounts of analyte to an unknown sample, a process known as spiking. By increasing the number of spikes, the analyst can extrapolate for the analyte concentration in the unknown that has not been spiked. [2] There are multiple approaches to the standard addition. The following section summarize each approach.
Silica gel particles are commonly used as a stationary phase in high-performance liquid chromatography (HPLC) for several reasons, [13] [14] including: High surface area: Silica gel particles have a high surface area, allowing direct interactions with solutes or after bonding of variety of ligands for versatile interactions with the sample molecules, leading to better separations.
The engineering design process, also known as the engineering method, is a common series of steps that engineers use in creating functional products and processes. The process is highly iterative – parts of the process often need to be repeated many times before another can be entered – though the part(s) that get iterated and the number of such cycles in any given project may vary.
Le Roux's research team assessed HPTLC for determining salbutamol serum levels in clinical trials and concluded that it is a suitable method for analyzing serum samples. [ 3 ] HPTLC has also been used successfully in the separation of various lipid subclasses, with reproducible and promising results obtained for 20 different lipid subclasses.