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Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.
Thermo Fisher Scientific's previous headquarters in Waltham, Massachusetts Thermo Fisher Scientific office in Canada. Thermo Fisher Scientific Inc. is an American life, science and clinical research company. It is a global supplier of analytical instruments, clinical development solutions, specialty diagnostics, laboratory, pharmaceutical and ...
A prime editing guide RNA (pegRNA), capable of (i) identifying the target nucleotide sequence to be edited, and (ii) encoding new genetic information that replaces the targeted sequence. The pegRNA consists of an extended single guide RNA (sgRNA) containing a primer binding site (PBS) and a reverse transcriptase (RT) template sequence.
A Guide to the Scientific Knowledge of Things Familiar, also known as The Guide to Science or Brewer's Guide to Science, is a book by Ebenezer Cobham Brewer presenting explanations for common phenomena. [1] First published in the United Kingdom around 1840, the book is laid out in the style of a catechism and proved very popular.
How To: Absurd Scientific Advice for Common Real-World Problems is a book by Randall Munroe in which the author provides absurd suggestions based in scientific fact on ways to solve some common and some absurd problems. [1] [2] [3] The book contains a range of possible real-world and absurd problems, each the focus of a single chapter. The book ...
Priming is the first contact that antigen-specific T helper cell precursors have with an antigen.It is essential to the T helper cells' subsequent interaction with B cells to produce antibodies. [1]
A protocol describes what types of people may participate in the trial; the schedule of tests, procedures, medications, and dosages; and the length of the study. While in a clinical trial, participants following a protocol are seen regularly by research staff to monitor their health and to determine the safety and effectiveness of their treatment.
Optimus Prime loses all memory of being a Prime after using the Matrix of Leadership to send Unicron back into stasis. Megatron takes advantage of this and takes "Orion"(Optimus's name before becoming a Prime) on to the Nemesis , convincing him the Decepticons are good and the Autobots are evil (Before becoming a Prime, Optimus and Megatron ...