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TRIzol reagent contains guanidinium thiocyanate and phenol.. TRIzol is a widely used [1] chemical solution used in the extraction of DNA, RNA, and proteins from cells. The solution was initially used and published by Piotr Chomczyński and Nicoletta Sacchi in 1987.
Phenol (also known as carbolic acid, phenolic acid, or benzenol) is an aromatic organic compound with the molecular formula C 6 H 5 OH. [5] It is a white crystalline solid that is volatile . The molecule consists of a phenyl group ( −C 6 H 5 ) bonded to a hydroxy group ( −OH ).
Phenol extraction is a laboratory technique that purifies nucleic acid samples using a phenol solution. Phenol is common reagent in extraction because its properties allow for effective nucleic acid extraction, particularly as it strongly denatures proteins, it is a nucleic acid preservative, and it is immiscible in water.
The best-selling drug in the U.S., Acetaminophen, also known as Paracetamol, is a phenol. There are various classification schemes. [15]: 2 A commonly used scheme is based on the number of carbons and was devised by Jeffrey Harborne and Simmonds in 1964 and published in 1980: [15]: 2 [16]
In female elephants, the two compounds 3-ethyl phenol and 2-ethyl 4,5 dimethylphenol have been detected in urine samples. [70] Temporal glands secretion examination showed the presence of phenol, m-cresol and p-cresol (4-methyl phenol) during musth in male elephants. [71] [72] [73] p-Cresol and o-cresol are also components of the human sweat.
Thymol (also known as 2-isopropyl-5-methylphenol, IPMP), C 10 H 14 O, is a natural monoterpenoid phenol derivative of p-Cymene, isomeric with carvacrol.It occurs naturally in the oil of thyme, and it is extracted from Thymus vulgaris (common thyme), ajwain, [4] and various other plants as a white crystalline substance of a pleasant aromatic odor and strong antiseptic properties.
The ferric chloride test is used to determine the presence of phenols in a given sample or compound (for instance natural phenols in a plant extract). Enols, hydroxamic acids, oximes, and sulfinic acids give positive results as well. [1]
Used in conjunction with phenol solutions, it results in an intense reaction that leads to initial skin sloughing. Since croton oil is very irritating and painful, it is used in laboratory animals to study how pain works, pain-relieving and anti-inflammatory drugs, and immunology. [4]