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Nicotinamide adenine dinucleotide phosphate, abbreviated NADP [1] [2] or, in older notation, TPN (triphosphopyridine nucleotide), is a cofactor used in anabolic reactions, such as the Calvin cycle and lipid and nucleic acid syntheses, which require NADPH as a reducing agent ('hydrogen source').
The actual concentration of NAD + in cell cytosol is harder to measure, with recent estimates in animal cells ranging around 0.3 mM, [18] [19] and approximately 1.0 to 2.0 mM in yeast. [20] However, more than 80% of NADH fluorescence in mitochondria is from bound form, so the concentration in solution is much lower.
Rather, NADP-ME was directly transformed from a C 3 species in photosynthesis, and even earlier origins from an ancient cystolic ancestor. In the cytosol, the enzyme existed as a series of housekeeping isoforms purposed towards a variety of functions including malate level maintenance during hypoxia, microspore separation, and pathogen defense.
One of the uses of NADPH in the cell is to prevent oxidative stress. It reduces glutathione via glutathione reductase, which converts reactive H 2 O 2 into H 2 O by glutathione peroxidase. If absent, the H 2 O 2 would be converted to hydroxyl free radicals by Fenton chemistry, which can attack the cell. Erythrocytes, for example, generate a ...
This is the second core process in photosynthesis. The initial stages occur within picoseconds, with an efficiency of 100%. The seemingly impossible efficiency is due to the precise positioning of molecules within the reaction center. This is a solid-state process, not a typical chemical reaction. It occurs within an essentially crystalline ...
The apicoplast organelle in these organisms is believed to have come from an endosymbiotic event in which an ancestral protozoan engulfed an algal cell. [7] These apicoplasts contain plant-like FNRs that the protozoan uses to generate reduced ferredoxin , which is then used as a reductant in essential biosynthetic pathways. [ 17 ]
The main role of these is to transport hydrogen atom to electron transport chain which will change ADP to ATP by adding one phosphate during metabolic processes (e.g. photosynthesis and respiration). Hydrogen carrier participates in an oxidation-reduction reaction [2] by getting reduced due to the acceptance of a Hydrogen.
Four different subunits were found to be important for the function of the photosynthetic reaction center. The L and M subunits, shown in blue and purple in the image of the structure, both span the lipid bilayer of the plasma membrane. They are structurally similar to one another, both having 5 transmembrane alpha helices. [6]