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The T-DNA is transferred from bacterium into the host plant's nuclear DNA genome. [1] The capability of this specialized tumor-inducing (Ti) plasmid is attributed to two essential regions required for DNA transfer to the host cell. The T-DNA is bordered by 25-base-pair repeats on each end.
The ABRC's mission is to acquire, preserve and distribute seed and DNA resources that are useful to the Arabidopsis research community. TAIR’s community includes over 28,000 registered users [ 4 ] and the website draws about 60,000 unique visitors per month.
Using primers for the reporter gene sections, the DNA can be amplified for sequencing. The process of cutting, self ligation and re cutting allows the amplification of the flanking regions of DNA without knowing the sequence. The point at which the ligation occurred can be seen by identifying the cut site of [enzyme 1].
Arabidopsis (rockcress) is a genus in the family Brassicaceae. They are small flowering plants related to cabbage and mustard . This genus is of great interest since it contains thale cress ( Arabidopsis thaliana ), one of the model organisms used for studying plant biology and the first plant to have its entire genome sequenced.
Arabidopsis thaliana is a first class model organism and the single most important species for fundamental research in plant molecular genetics. A. thaliana was the first plant for which a high-quality reference genome sequence was determined and a worldwide research community has developed many other genetic resources and tools.
The site of T-DNA insertions has been determined for over 300,000 independent transgenic lines, with the information and seeds accessible through online T-DNA databases. [49] Through these collections, insertional mutants are available for most genes in A. thaliana.
The design of appropriate short or long primer pairs is only one goal of PCR product prediction. Other information provided by in silico PCR tools may include determining primer location, orientation, length of each amplicon , simulation of electrophoretic mobility, identification of open reading frames , and links to other web resources.
The only essential parts of the T-DNA are its two small (25 base pair) border repeats, at least one of which is needed for plant transformation. [24] [25] The genes to be introduced into the plant are cloned into a plant transformation vector that contains the T-DNA region of the plasmid. An alternative method is agroinfiltration. [26] [27]