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Horseradish peroxidase is a 44,173.9-dalton glycoprotein with six lysine residues which can be conjugated to a labeled molecule. It produces a coloured, fluorimetric [6] or luminescent derivative of the labeled molecule when incubated with a proper substrate, allowing it to be detected and quantified.
Peroxidase can be used for treatment of industrial waste waters. For example, phenols, which are important pollutants, can be removed by enzyme-catalyzed polymerization using horseradish peroxidase. Thus phenols are oxidized to phenoxy radicals, which participate in reactions where polymers and oligomers are produced that are less toxic than ...
TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase.. Shows the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to 3,3',5,5'-tetramethylbenzidine diimine
Horseradish peroxidase is commonly linked to secondary antibodies to allow the detection of the target protein by chemiluminescence. The chemiluminescent substrate is cleaved by horseradish peroxidase, resulting in the production of luminescence. Therefore, the production of luminescence is proportional to the amount of horseradish peroxidase ...
When in 1981 Rob Aalberse from the University of Amsterdam noticed the enormous cross-reactivity of some patients´ sera against virtually any plant and even insects, notably, insect venoms, [1] it took ten years to arrive at a possible structural explanation of this phenomenon. 1991, Japanese researchers determined the structure of the epitope common to horseradish peroxidase and Drosophila ...
If horseradish peroxidase (HRP) is going to be used, the sample must be incubated in hydrogen peroxide to suppress endogenous peroxidase activity. [11] If digoxigenin was used as a probe label, an anti-digoxigenin fluorescein primary antibody followed by a HRP-conjugated anti-fluorescein secondary antibody are then applied. [1]
“Once the cause is identified, the doctor will suggest an appropriate treatment,” Dr. Nocerino says. This may include medications, swallowing therapy, surgery or endoscopic treatments.
Eclox ECL is a broadband enhanced chemiluminescence (ECL) assay that can be used to qualitatively assess a water sample to determine whether it has been contaminated. ECL reactions are used in a number of clinical and analytical applications and are based upon the oxidation of luminol in the presence of the enzyme horseradish peroxidase (HRP), an oxygen source, and an enhancer such as 4 ...