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Just like baking soda and vinegar simulate a volcanic eruption, baking soda interacts with acidic ingredients in doughs and batters to create bubbles of CO 2. But instead of spilling out of a ...
Into a second flask, add charcoal, yeast extract, alpha-keto-glutarate, and agar. Mix the dry powders. Pour the buffer solution into the second flask containing the dry powders and mix. Carefully heat to dissolve the agar, then sterilize by autoclaving at 121 °C for 15 minutes. Immediately place the medium in 50 °C water bath.
As the gelatin cools, these bonds try to reform in the same structure as before, but now with small bubbles of liquid in between. This gives gelatin its semisolid, gel-like texture. [20] Because gelatin is a protein that contains both acid and base amino groups, it acts as an amphoteric molecule, displaying both acidic and basic properties.
YM Agar and Broth, is a selective growth medium with low pH useful for cultivating yeasts, molds, or other acid-tolerant or acidophilic organisms, while deterring growth of most bacteria and other acid intolerant organisms. [1] It is malt extract medium modified by the addition of yeast extract and peptone. [2]
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TCBS agar contains high concentrations of sodium thiosulfate and sodium citrate to inhibit the growth of Enterobacteriaceae. Inhibition of gram-positive bacteria is achieved by the incorporation of ox gall , which is a naturally occurring substance containing a mixture of bile salts and sodium cholate , a pure bile salt .
Carrageenan is a vegetarian and vegan alternative to gelatin in some applications, and is used to replace gelatin in confectionery and other food. The first industrial commercial cultivation of Eucheuma and Kappaphycus spp. for carrageenan was developed in the Philippines. The global top producers of carrageenan are the Philippines and Indonesia.
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.