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The design of appropriate short or long primer pairs is only one goal of PCR product prediction. Other information provided by in silico PCR tools may include determining primer location, orientation, length of each amplicon, simulation of electrophoretic mobility, identification of open reading frames, and links to other web resources. [7] [8] [9]
A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.
Agarose Gel simulation. Online Database searching - Search public databases at the NCBI such as Genbank and UniProt. Online BLAST searches. Protein analysis including estimation of Molecular Weight, Extinction Coefficient and pI. PCR Primer design, including an interface to Primer3; 3D structure viewing via an interface to Jmol
Viability PCR workflow. Viability PCR, also named v-PCR or vPCR, is an evolution of PCR. Through the use of a simple pre-treatment of the sample by the means of specific intercalating photo-reactive reagents it's possible to neutralize the DNA of dead cells. As a result, only DNA from live cells will be detected by PCR.
Schrödinger's software tools include molecular dynamics simulations, free energy calculations, quantum mechanics calculations, and virtual screening tools. The company also offers consulting services and collaborates with partners in the industry to advance the field of computational chemistry and drug discovery.
Virtual - A simulation involving real people operating simulated systems. Virtual simulations inject a Human-in-the-Loop into a central role by exercising motor control skills (e.g., flying jet or tank simulator), decision making skills (e.g., committing fire control resources to action), or communication skills (e.g., as members of a C4I team).
A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR.
Virtual Cell is an advanced software platform for modeling and simulating reaction kinetics, membrane transport and diffusion in the complex geometries of cells and multicellular tissues. VCell models have a hierarchical tree structure.