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When the molecule returns to the ground state, it releases a photon, which is the fluorescent emission. The excited state lifetime is short, so emission of light is typically only observable when the absorbing light is on. Fluorescence can be of any wavelength but is often more significant when emitted photons are in the visible spectrum.
The spectrum of light emitted from a fluorescent lamp is the combination of light directly emitted by the mercury vapor, and light emitted by the phosphorescent coating. The spectral lines from the mercury emission and the phosphorescence effect give a combined spectral distribution of light that is different from those produced by incandescent ...
The fluorescent light is emitted in all directions. Some of this fluorescent light passes through a second filter or monochromator and reaches a detector, which is usually placed at 90° to the incident light beam to minimize the risk of transmitted or reflected incident light reaching the detector.
The luminous intensity (in candelas) is a measure of how bright the beam in a particular direction is. If a lamp has a 1 lumen bulb and the optics of the lamp are set up to focus the light evenly into a 1 steradian beam, then the beam would have a luminous intensity of 1 candela. If the optics were changed to concentrate the beam into 1/2 ...
The fluorophore absorbs light energy of a specific wavelength and re-emits light at a longer wavelength. The absorbed wavelengths, energy transfer efficiency, and time before emission depend on both the fluorophore structure and its chemical environment, since the molecule in its excited state interacts with surrounding molecules.
Fluorometer designed to measure chlorophyll fluorescence in plants. A fluorometer, fluorimeter or fluormeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. [1]
Standards FL1–FL6 represent "standard" fluorescent lamps consisting of two semi-broadband emissions of antimony and manganese activations in calcium halophosphate phosphor. FL4 is of particular interest since it was used for calibrating the CIE color rendering index (the CRI formula was chosen such that FL4 would have a CRI of 51). [citation ...
Calcein, also known as fluorexon, fluorescein complex, is a fluorescent dye with excitation and emission wavelengths of 495 and 515 nm, respectively, and has the appearance of orange crystals. Calcein self-quenches at concentrations above 70 mM and is commonly used as an indicator of lipid vesicle leakage.