Ads
related to: proper pipette technique pdf free
Search results
Results From The WOW.Com Content Network
Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. The technique is mainly recommended for solutions with a high viscosity or a tendency to foam: [ 1 ] as it reduces the risk of splashing, foam or bubble formation.
Forward pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. The technique is mainly recommended for aqueous solutions, such as buffers, or diluted acids or alkalis. In case of solutions with a high viscosity or a tendency to foam, reverse pipetting is more suitable.
Winged elbow pipetting Technique: elevated, “winged elbow”. The average human arm weighs approximately 6% of the total body weight. Holding a pipette with the elbow extended (winged elbow) in a static position places the weight of the arm onto the neck and shoulder muscles and reduces blood flow, thereby causing stress and fatigue.
Whereas manual pipettes need a movement of the thumb (up to 3 cm), electronic pipettes have a main button. The programming of the pipette is generally done by a control wheel and some further buttons. All settings are displayed on a small display. Electronic pipettes can decrease the risk of RSI-type injuries. [8] [9]
Positive displacement pipettes are a type of pipette that operates via piston-driven displacement. [1] [2] Unlike an air displacement pipette, which dispenses liquid using an air cushion in the pipette tip, the piston in a positive displacement pipette makes direct contact with the sample, allowing the aspiration force to remain constant.
In developmental, cell and molecular biology, trituration is the process of fragmenting of solid material (often biological tissue or aggregated material) into smaller components (often, respectively, cells or molecules in suspension/solution) by means of repeated passage through a pipette.
A Mohr pipette, also known as a graduated pipette, is a type of pipette used to measure the volume of the liquid dispensed, although not as accurately as a volumetric pipette. [1] These use a series of marked lines (as on a graduated cylinder) to indicate the different volumes. [ 2 ]
A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 millilitres (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz). [2]
Ad
related to: proper pipette technique pdf free