Ads
related to: in vivo luciferase assay protocol
Search results
Results From The WOW.Com Content Network
Renilla-luciferin 2-monooxygenase, Renilla luciferase, or RLuc, is a bioluminescent enzyme found in Renilla reniformis, belonging to a group of coelenterazine luciferases.Of this group of enzymes, the luciferase from Renilla reniformis has been the most extensively studied, and due to its bioluminescence requiring only molecular oxygen, has a wide range of applications, with uses as a reporter ...
The sensitivity and high signal intensity of this luciferase molecule proves advantageous in many reporter studies. Some of the benefits of using a secreted reporter molecule like MetLuc is its no-lysis protocol that allows one to be able to conduct live cell assays and multiple assays on the same cell. [8]
[4] [5] These qualities make RVPs a safer and faster alternative to plaque assays, and especially well-suited for high-throughput applications. [ 4 ] [ 5 ] [ 9 ] [ 11 ] [ 12 ] RVPs offer flexibility for different uses, as they are antigenically identical to wild-type virus, and can be engineered with various proteins or express mutant envelopes ...
Bioluminescence emitted from colonies of microbial cells containing the genes for bacterial luciferase. Luciferase is a generic name for an enzyme that catalyzes a light-emitting reaction. Luciferases can be found in bacteria, algae, fungi, jellyfish, insects, shrimp, and squid, and the resulting light that these organisms produce is termed ...
It consists of a modified cell line that has been stably transfected with a DNA construct with a luciferase reporter gene under control of receptor-specific DNA response elements that can stimulate transcription of the inserted luciferase gene and produce the light-generating enzyme which can be easily measured. The DNA response elements can be ...
Reporter genes can be used to assay for the activity of a particular promoter in a cell or organism. [23] In this case there is no separate "gene of interest"; the reporter gene is simply placed under the control of the target promoter and the reporter gene product's activity is quantitatively measured.