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The KOH test, also known as a potassium hydroxide preparation or KOH prep, is a quick, inexpensive fungal test to differentiate dermatophytes and Candida albicans symptoms from other skin disorders like psoriasis and eczema. [1] Dermatophytes are a type of fungus that invades the top layer of the skin
Vaginal wet mount showing slings of pseudohyphae of Candida albicans surrounded by round vaginal epithelial cells, conferring a diagnosis of candidal vulvovaginitis.. A vaginal wet mount (or vaginal smear [1] or wet prep [2]) is a gynecologic test wherein a sample of vaginal discharge is observed by wet mount microscopy by placing the specimen on a glass slide and mixing with a salt solution. [1]
The basis for the diagnosis of kerion is clinical finding, positive microscopic examinations (such as positive KOH preparation, Lactophenol cotton blue wet mount, Chicago sky blue stained (CSB) slide, Calcofluor white stained slide, Periodic acid–Schiff stained slide, and Gomori’s methenamine silver stained slide), mycological culture and modern molecular tests (such as PCR-reverse line ...
Potassium hydroxide (KOH) is added to the slide and the sample is examined with a microscope to determine presence of hyphae. [3] Care should be taken in procurement of a sample, as false-negative results may occur if the patient is already using an antifungal, if too small a sample is obtained, or if sample from a wrong site is collected.
Superficial scrapes of skin examined underneath a microscope may reveal the presence of a fungus.This is done by utilizing a diagnostic method called KOH test, [6] wherein the skin scrapings are placed on a slide and immersed on a dropful of potassium hydroxide solution to dissolve the keratin on the skin scrappings thus leaving fungal elements such as hyphae, septate or yeast cells viewable.
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In a comparative study of KOH, Calcofluor-White and Fungal cultures for diagnosing Fungal Onychomycosis, CFW was seen as the most sensitive method in detecting the infection in humans. The study saw that out of 150 patients CFW showed positive in 95 of them. The study also reviewed key advantages for using the Calcofluor-White stain over KOH.
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