Ads
related to: single molecule fluorescence imaging system
Search results
Results From The WOW.Com Content Network
Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy , imaging probes, and spectroscopy .
Single-molecule fluorescence spectroscopy uses the fluorescence of a molecule for obtaining information on its environment, structure, and position. The technique affords the ability of obtaining information otherwise not available due to ensemble averaging (that is, a signal obtained when recording many molecules at the same time represents an ...
Single molecule fluorescent sequencing is one method of DNA sequencing. The core principle is the imaging of individual fluorophore molecules, each corresponding to one base. [1] By working on single molecule level, amplification of DNA is not required, avoiding amplification bias. The method lends itself to parallelization by probing many ...
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide.
Photobleaching is an important parameter to account for in real-time single-molecule fluorescence imaging in biophysics. At light intensities used in single-molecule fluorescence imaging (0.1-1 kW/cm 2 in typical experimental setups), even most robust fluorophores continue to emit for up to 10 seconds before photobleaching in a single step. For ...
The system temporarily silences (sets in an OFF-state) all but one molecule within a diffraction-limited area (DLA) and then locates that single active (in an ON-state) molecule. [1] Super-resolution microscopy techniques like stochastic optical reconstruction microscopy (STORM) and photoactivated localization microscopy (PALM) do the same. [6]
Ads
related to: single molecule fluorescence imaging system