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A primary advantage of using ex vivo tissues is the ability to perform tests or measurements that would otherwise not be possible or ethical in living subjects. Tissues may be removed in many ways, including in part, as whole organs, or as larger organ systems. [citation needed] Examples of ex vivo specimen use include: [citation needed]
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century.
Endomicroscopy is a technique for obtaining histology-like images from inside the human body in real-time, [1] [2] [3] a process known as ‘optical biopsy’. [4] [5] It generally refers to fluorescence confocal microscopy, although multi-photon microscopy and optical coherence tomography have also been adapted for endoscopic use.
These advantages of three-photon microscopy gives it an edge in visualize in vivo and ex vivo tissue morphology and physiology at a cellular level deep within scattering tissue [4] and Rapid volumetric imaging. [17] In the recent study, Xu has demonstrated the potential of three-photon imaging for noninvasive studies of live biological systems ...
Biophotonics can also be described as the "development and application of optical techniques, particularly imaging, to the study of biological molecules, cells and tissue". [2] One of the main benefits of using the optical techniques which make up biophotonics is that they preserve the integrity of the biological cells being examined. [3] [4]
In systems biology, live single-cell imaging is a live-cell imaging technique that combines traditional live-cell imaging and time-lapse microscopy techniques with automated cell tracking and feature extraction, drawing many techniques from high-content screening. It is used to study signalling dynamics and behaviour in populations of ...
Optical imaging, using light as an investigational tool for biological research and medical diagnosis; Optoacoustic imaging, using the photothermal effect, for the accuracy of spectroscopy with the depth resolution of ultrasound; Photoacoustic Imaging, a technique to detect vascular disease and cancer using non-ionizing laser pulses
More recently, advancements in the use of protein-based imaging scaffolds are helping to solve the problems of sample orientation bias and size limit. Proteins smaller than ~50 kDa generally have too low a signal-to-noise ratio (SNR) to be able to resolve protein particles in the image, making 3D reconstruction difficult or impossible. [13]