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Multiple probe designs may be useful in identifying extraneous factors which may be influencing your results. Lastly, experimenters should avoid gathering data during sessions alone. If in-session data is gathered a note of the dates should be tagged to each measurement in order to provide an accurate time-line for potential reviewers.
Multiplex ligation-dependent probe amplification (MLPA) is a variation of the multiplex polymerase chain reaction that permits amplification of multiple targets with only a single primer pair. [1] It detects copy number changes at the molecular level, and software programs are used for analysis.
The design of the molecular inversion probes (MIP) originated from padlock probes, a molecular biology technique first reported by Nilsson et al. in 1994 . [7] Similar to MIP, padlock probes are single stranded DNA molecules with two 20-nucleotide long segments complementary to the target connected by a 40-nucleotide long linker sequence.
Multi-tip STM is used as a method for the detection of the spin-voltage in topological insulators using spin-polarized four-probe scanning tunneling microscopy on Bi 2 Te 2 Se surfaces. The spin-dependent electrochemical potential is separated from the ohmic contribution.
Multiplex-PCR consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple sequences at once, additional information may be gained from a single test run that otherwise would require several times the reagents and more time to perform.
Repeated measures design is a research design that involves multiple measures of the same variable taken on the same or matched subjects either under different conditions or over two or more time periods. [1] For instance, repeated measurements are collected in a longitudinal study in which change over time is assessed.
Biofilms, for example, are composed of complex (often) multi-species bacterial organizations. Preparing DNA probes for one species and performing FISH with this probe allows one to visualize the distribution of this specific species within the biofilm.
The carryover ratio is the percentage of H3 carry to L1 constituting the carryover portion "h". In a design of 3 high samples followed by 3 low samples, h can be calculated as (L1 - mean of L2&L3) / (H3 - mean of L2&L3) The carryover ratio's acceptance criteria depend on the measurement and the laboratory concerned.